RPS6KA3 polyclonal antibody
产品名称： RPS6KA3 polyclonal antibody
英文名称： RPS6KA3 polyclonal antibody
产品编号： PAB2314
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of RPS6KA3.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to amino acids 382-411 at C-terminus of human RPS6KA3.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- RSK2 activity is regulated by its interaction with PEA-15.
Vaidyanathan H, Ramos JW.J Biol Chem. 2003 Aug 22;278(34):32367-72. Epub 2003 Jun 9.
- 2.
- Gene diversity patterns at 10 X-chromosomal loci in humans and chimpanzees.
Kitano T, Schwarz C, Nickel B, Paabo S.Mol Biol Evol. 2003 Aug;20(8):1281-9. Epub 2003 May 30.
- 3.
- Ataxia telangiectasia mutated proteins, MAPKs, and RSK2 are involved in the phosphorylation of STAT3.
Zhang Y, Cho YY, Petersen BL, Bode AM, Zhu F, Dong Z.J Biol Chem. 2003 Apr 11;278(15):12650-9. Epub 2003 Jan 31.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of RPS6KA3 polyclonal antibody (Cat # PAB2314) in HL-60 cell lysate. RSK2 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the RPS6KA3 polyclonal antibody (Cat # PAB2314) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 6197
- Protein Accession#:
- P51812
- Gene Name:
- RPS6KA3
- Gene Alias:
- CLS,HU-3,ISPK-1,MAPKAPK1B,MRX19,RSK,RSK2,S6K-alpha3,p90-RSK2,pp90RSK2
- Gene Description:
- ribosomal protein S6 kinase, 90kDa, polypeptide 3
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq
- Other Designations:
- OTTHUMP00000023036,insulin-stimulated protein kinase 1,mental retardation, X-linked 19,ribosomal protein S6 kinase, 90kD, polypeptide 3